Reproducibility of GMP-compliant production of therapeutic stressed peripheral blood mononuclear cell-derived secretomes, a novel class of biological medicinal products

Table 4 Protein quantification in large PBMC secretome batches. < LOD below limit of detection

Sample	PAI-1 (ng/mL)	MMP9 (ng/mL)	EGF (pg/mL)	TGFβ1 (ng/mL)	IL-8 (ng/mL)	Calprotectin (ng/mL)
PBMC^sec 1	1.98	438	113	4.75	3.41	31
PBMC^sec 2	1.64	639	89	2.25	3.87	27
PBMC^sec 3	1.44	600	100	2.79	1.68	29
Placebo	< LOD	< LOD	< LOD	< LOD	< LOD	< LOD
Mean PBMC^sec batches	1.69	559	100.7	3.26	2.98	29
SD PBMC^sec batches	0.23	87	9.8	1.08	0.94	1.4
% deviation PBMC^sec batches	13.3	15.6	9.7	33.0	31.6	4.9

ISSN: 1757-6512