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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Long noncoding RNA HULC accelerates the growth of human liver cancer stem cells by upregulating CyclinD1 through miR675-PKM2 pathway via autophagy

Fig. 7

CyclinD1 is required for HULC oncogenic action. a RT-PCR for HULC and Western blotting with anti-CyclinD1. β-actin served as internal control. b Cell growth assay using CCK8. c Colony formation assay. d The picture of xenografted tumors from mouse. e The wet weight of xenografted tumors from mouse. f The appearance time of xenograft tumor. g PCNA staining (DAB staining, original magnification× 100). h PCNA positive rate analysis

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