Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells

Fig. 3

Blocking the D1 receptor inhibits hBMSC osteogenic differentiation. a Histochemical staining and b total absorbance measurements of ALP during early hBMSC osteogenic differentiation stimulated with DA, DA+D1 antagonist (SCH-23390) and DA+ D2 antagonist (haloperidol) (n = 3 for all groups). c Alizarin Red S staining and d total absorbance measurements during late hBMSC osteogenic differentiation stimulated with DA, DA+SCH-23390 and DA+ haloperidol (n = 3 for all groups). Quantitative RT-PCR analysis of osteogenic gene expression during hBMSC osteogenic differentiation after transfection with D1 receptor-specific (e) or D2 receptor-specific (f) siRNA (n = 3 for all groups). The results are shown as the mean ± standard error. Statistical significance was assessed by unpaired Student’s t test or one-way ANOVA test for multiple-group comparisons; *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page