Fig. 5From: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cellsThe activation of the D1 receptor enhances ERK1/2 phosphorylation and facilitates hBMSC osteogenic differentiation by increasing Runx2 transcriptional activity. a Immunoblot analysis of Runx2, phosphorylation, and total ERK1/2, p38 MAPK, and JNK expression during hBMSC osteogenic differentiation stimulated with SKF-38393 and pramipexole (n = 3 for all groups). b ChIP assay analysis of Runx2 transcriptional activity in bonding with ALP, BSP, OCN, and OSX promoter during hBMSC osteogenic differentiation stimulated with SKF-38393 (n = 3 for all groups). The results are shown as the mean ± standard error. Statistical significance was assessed by unpaired Student’s t test or one-way ANOVA test for multiple-group comparisons; *P < 0.05; **P < 0.01; ***P < 0.001Back to article page