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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Dopamine D1 receptor-mediated activation of the ERK signaling pathway is involved in the osteogenic differentiation of bone mesenchymal stem cells

Fig. 6

Blocking the ERK1/2 signaling pathway inhibits hBMSC osteogenic differentiation. a Histochemical staining and b total absorbance measurements of ALP during early hBMSC osteogenic differentiation stimulated with SKF-38393, ERK inhibitor (U-0126), and SKF-38393+ U-0126 (n = 3 for all groups). c Alizarin Red S staining and d total absorbance measurements during late hBMSC osteogenic differentiation stimulated with SKF-38393, U-0126, and SKF-38393+ U-0126 (n = 3 for all groups). e Quantitative RT-PCR analysis of osteogenic gene expression during hBMSC osteogenic differentiation stimulated with SKF-38393, U-0126, and SKF-38393+ U-0126 (n = 3 for all groups). f Immunoblot analysis of Runx2, phosphorylation, and total ERK1/2 expression during hBMSC osteogenic differentiation stimulated with SKF-38393, U-0126, and SKF-38393+ U-0126 (n = 3 for all groups). g ChIP assay analysis of Runx2 transcriptional activity in bonding with ALP, BSP, OCN, and OSX promoter during hBMSC osteogenic differentiation stimulated with SKF-38393, U-0126, and SKF-38393+ U-0126 (n = 3 for all groups). The results are shown as the mean ± standard error. Statistical significance was assessed by one-way ANOVA test; *P < 0.05; **P < 0.01; ***P < 0.001

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