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Table 4 List of primers used in the luciferase reporter assay

From: Epigenetic regulation of IFITM1 expression in lipopolysaccharide-stimulated human mesenchymal stromal cells

Name Forward sequence (5′-3′) Reverse sequence (5′-3′) Location Size (bp)
R1 (Kpn1-Xho1) AAAggtaccTCTATCCGTCAGACAGAAACTCC ATActcgagGGACACTATCAAGGGGCTGA chr11:269,146–269,939 794
R2 (Kpn1-Xho1) AAAggtaccGCCAGCTGATCCATTGAGTA ATActcgagTTTATAGATCAAGGCTGAACAAGC chr11:311,846–312,080 235
R3 (promoter, Bgl ll-Hind III) ATAagatctAACACACTACCCTCTGGGGA ATAaagcttGTTTTCTGCGTGGAGCGAAG chr11:313,486–314,122 637
R6 (Kpn1-Xho1) AAAggtaccAGGGAACAGATGGCTCAGAA ATActcgagTCTCCGTTTCCCCGAAGTGA chr11:355,400–356,431 1032
R6 (Xho1-Kpn1) ATActcgagAGGGAACAGATGGCTCAGAA AAAggtaccTCTCCGTTTCCCCGAAGTGA chr11:355,400–356,431 1032