Fig. 2From: Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageingNTA study of MSC-derived EVs at several ages. a Representative results from the NTA assay of old and young MSC-derived EVs. b Electron micrographs of MSC-derived EVs from the old and young groups of rats (scale bar = 100 nm). c The number of particles per cell at different age groups by NTA assay. d Concentration of protein per particle at different age groups as determined by NTA assay. e Mean size of particles, expressed in nanometres, for different age groups as determined by NTA assay. *P < 0.05 compared with the former group was considered statistically significant using Mann-Whitney U and Kruskal-Wallis tests. f Ponceau S staining for protein content for 20 μg MSC-derived EVs and their immunoblot staining for exosome markers CD63, TSG101 and CalnexinBack to article page