Fig. 5
From: Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageing
Study of senescence in MSCs. a The histograms represent the gene expression of LMNA normalised by HPRT, a housekeeping gene, in old and young MSCs with or without young or old MSC-derived EVs. *P < 0.05 compared with the control was considered statistically significant using Mann-Whitney U and Kruskal-Wallis tests. Control, MSCs cultured with growth medium without EVs; oMSC, MSCs from the old group; yMSC, MSCs from the young group; oEV, MSC-derived EVs from the old group; yEV, MSC-derived EVs from the young group. b Western blot analysis of pre-lamin A and their isoforms, lamin A/C, from old and young MSCs with or without old or young MSC-derived EVs at different times (2, 3 and 6 days) and LMNA densitometry analysis normalised with respect to β-actin, using Image Quant 5.2. The molecular weight of each protein is shown on the left. One representative experiment is shown. The gels have been run under the same experimental conditions