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Table 1 Specific primers for real-time reverse transcriptase polymerase chain reaction (RT-PCR) amplification, listed with their annealing temperature (AT)

From: Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageing

Gene nameFw primerRv primermRNA IDAT (°C)
NanogatgcctcacacggagactgtaagtgggttgtttgcctttgNM_005103.461
Oct4ctcctggagggccaggaatcatatacacaggccgatgtggNM_0051061
VinculinaggagaccttgcgaagacagggcggttgccacttgtttagNM_00110724861
LMNAgagcaaagtgcgtgaggagttccccctccttcttggtattNM_001002016.261
mTORatcaagcaagcgacatctcacaggccttggttaccagaaaNM_019906.161
AKTgacgtagccattgtgaaggagccatcattcttgaggaggaagtNM_033230.261
RictorgttggaaaaatggcacaaggctgtatgtagtgagggcttcgttNC_005101.461
HPRTagccgaccggttctgtcatagccgaccggttctgtcaNM_012583.261
  1. Fw forward, Rv reverse