Fig. 7
In vitro studies on ASCs seeded PCL scaffolds: a, b Representative images of ASCs seeded into PCL after 21 days of culture. Alizarin red staining and mineralization assays of rat ASCs after 21 days: a control, non-differentiation culture of rat ASCs, b osteogenic differentiation. Seeding density of ASCs: 0.9 × 106/scaffold. Representative images are shown at × 10 magnification. Scale bars represent 100 μm. Images were obtained using an Olympus CKX41 microscope. c Typical μCT images of the PCL and scaffolds seeded with ASCs and incubated for 21 days. The images were reconstructed and analysed using the SkyScan NRecon and CtAn software. The 3D images were generated using SkyScan CTvol