Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Protective properties of heme oxygenase-1 expressed in umbilical cord mesenchymal stem cells help restore the ovarian function of premature ovarian failure mice through activating the JNK/Bcl-2 signal pathway-regulated autophagy and upregulating the circulating of CD8+CD28− T cells

Fig. 1

Identification of UCMSCs and GCs, and the transduction efficiency of the HO-1/shHO-1 plasmids into UCMSCs. a Black histograms represent expression of indicated cell surface marker. b Cultured UCMSCs show fibroblast-like morphology (× 100). c, d UCMSCs cultured under conditions for differentiation into osteoblasts or lipoblasts. Osteoblasts are displayed by Alizarin Red staining and darker red staining indicates calcium deposition (× 200, c). Lipoblasts displayed by accumulation of neutral lipid vacuoles stained with Oil Red O (× 400, d). e, f Morphology and phenotypes of GCs. Cultured GCs show spindle-shaped morphology (× 100, e). Blue staining indicates the GCs’ nucleus; brown staining indicates FSHR-positive expression in cytoplasm (× 400, f). g The transfection efficiency of the HO-1/shHO-1 plasmids into UCMSCs. *P < 0.05, ***P < 0.001 vs NC group. GCs, granulosa cells; HO-1, heme oxygenase-1; UCMSCs, umbilical cord mesenchymal stem cells

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512