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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Protective properties of heme oxygenase-1 expressed in umbilical cord mesenchymal stem cells help restore the ovarian function of premature ovarian failure mice through activating the JNK/Bcl-2 signal pathway-regulated autophagy and upregulating the circulating of CD8+CD28 T cells

Fig. 7

Induction of autophagy in GCs in response to HO-1/shHO-1-MSCs, treated with or without anisomycin or SP600125. A-1–A-9 HN2-treated GCs co-cultured with UCMSCs transfected with HO-1/shHO-1 plasmids with or without the administration of SP600125 or anisomycin for 48 h in vitro. Representative fluorescent and phase-contrast images of the treated cells were shown. A-1 GC group. A-2 HN2 group. A-3 MSC group. A-4 NC group. A-5 HO-1 group. A-6 shHO-1 group. A-7 DMSO group. A-8 SP600125 group. A-9 Anisomycin group. A-10 Fluorescence intensity analysis of GCs in each group. B1B9 POF mice transplanted with HO-1/shHO-1-MSCs and administrated with SP600125 or anisomycin for 48 h and imaged by TEM in vivo. Representative images of cells are shown as B1 control group. B2 POF group. B3 MSC group. B4 NC group. B5 HO-1 group. B6 shHO-1 group. B7 DMSO group. B8 Anisomycin group. B9 SP600125 group. White arrows indicate accumulation of autophagosome; black arrows indicate chromatin condensation. × 1900 (a), bar scale = 5 μm. × 4800 (b), bar scale = 2 μm. *P < 0.05, **P < 0.01, ***P < 0.001 vs POF, NC group, or DMSO group, separately. DMSO, dimethylsulfoxide; GCs, granulosa cells; HN2, chlormethine; HO-1, heme oxygenase-1; NC, empty vector; POF, premature ovarian failure; UCMSCs, umbilical cord mesenchymal stem cells

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