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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: hUCMSC-extracellular vesicles downregulated hepatic stellate cell activation and reduced liver injury in S. japonicum-infected mice

Fig. 1

Characteristics of hUCMSCs and hUCMSC-EVs. Morphology of a passage 0 and b passage 3 hUCMSCs were observed under light microscopy (× 100). Representative images of osteocyte (× 100) and chondrocyte (× 200) differentiation of hUCMSCs cultured in the differentiation medium. The cells were stained with c Alizarin Red and d Alcian Blue. e Results for the flow cytometry analyses of phenotypic markers related to MSCs. f hUCMSC-EVs were observed under a transmission electron microscope; some of the hUCMSC-EVs are indicated by arrows. Scale bar = 200 nm. g Size distributions of hUCMSC-EVs were detected using the NTA. h Western blotting analysis of CD63 and Calreticlin expression in lysates from hUCMSC-EVs and hUCMSCs. i Flow cytometry detection of CD73 and CD90. EVs reacted with the isotype antibody were applied as the negative control (NC)

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Stem Cell Research & Therapy

ISSN: 1757-6512