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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling

Fig. 3

The effect of endogenous ephrinB2 overexpression on osteogenic differentiation of hDPSCs. a ALP staining on day 7 of osteogenic differentiation. Scale bar = 200 μm. Staining intensity was quantified with ImageJ. b Alizarin Red S staining on days 14, 21, and 28 of osteogenesis. Staining intensity was quantified with ImageJ. ce mRNA expression of RUNX2, ALP, BMP2, COL1, OCN, DSPP, and DMP1 in EfnB2-hDPSCs (ephrinB2 overexpression) and Vector-hDPSCs (ephrinB2 overexpression control) after 7, 14, and 21 days of osteogenic induction. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 and **p < 0.01 vs. Vector-hDPSCs

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