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Table 1 The sequences of canine and human primers used in qRT-PCR

From: EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling

SpeciesGenePrimers
Homo sapiensephrinB2For: 5′-TATGCAGAACTGCGATTTCCAA-3′
Rev: 5′-TGGGTATAGTACCAGTCCTTGTC-3′
ALPFor: 5′-CCTCGTTGACACCTGGAAGAG-3′
Rev: 5′-TTCCGTGCGGTTCCAGA-3′
RUNX2For: 5′-TCTTAGAACAAATTCTGCCCTTT-3′
Rev: 5′-TGCTTTGGTCTTGAAATCACA-3′
BMP2For: 5′-TTCCACCATGAAGAATCTTTGGA-3′
Rev: 5′-CCTGAAGCTCTGCTGAGGTGAT-3′
COL1For: 5′-GAGGGCCAAGACGAAGACATC-3′
Rev:5′- CAGATCACGTCATCGCACAAC-3′
OCNFor: 5′-CTACCTGTATCAATGGCTGGG-3′
Rev: 5′-GGATTGAGCTCACACACCT-3′
DSPPFor: 5′-TTTGGGCAGTAGCATGGGC-3′
Rev: 5′-CCATCTTGGGTATTCTCTTGCCT-3′
DMP1For: 5′-CTCCGAGTTGGACGATGAGG-3′
Rev: 5′-TCATGCCTGCACTGTTCATTC-3′
EphB4For: 5′-CGCACCTACGAAGTGTGTGA-3′
Rev: 5′-GTCCGCATCGCTCTCATAGTA-3′
β-actinFor: 5′-ACGTTGCTATCCAGGCTGTG-3′
Rev: 5′-GGCCATCTCTTGCTCGAAGT-3′
Canis familiarisRUNX2For: 5′-TACCACACCTACCTGCCACCAC-3′
Rev: 5′-GCGGAAGCATTCTGGAAGGAGAC-3′
BMP2For: 5′-TGAACTCCACTAACCACGCCATTG-3′
Rev: 5′-TGTTGGTACACAGCACGCCTTG-3′
ephrinB2For: 5′-TGCCAGACAAGAGCCATGAAGATC-3′
Rev: 5′-GGCGTCGTGTTGGATCATTATGC-3′
β-actinFor: 5′-ATCACTATTGGCAACGAGCGGTTC-3′
Rev: 5′-CAGCACTGTGTTGGCATAGAGGTC-3′
  1. Abbreviations: ALP alkaline phosphatase, RUNX2 runt-related transcription factor 2, BMP2 bone morphogenetic protein 2, COL1 collagen type I, OCN osteocalcin, DSPP dentin sialophosphoprotein, DMP1 dentin matrix protein 1, For forward, Rev reverse