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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair

Fig. 4

Engraftment of LGSC allotransplantation and relief of ADDED symptoms. a–f Immunohistochemistry (IHC) staining of NOD/ShiLtJ LG transplanted with ROSA-LGSCs cultured for 7 days after 8 weeks. a–c IHC staining with anti-td-Tomato antibody; d–f IHC staining with anti-AQP5 antibody; a, d LG injected with vehicle, b, e LG injected with ROSA-LGSCs, c, f the magnified image of the black frame in b, e (red arrow, intralobular duct); scale bar, 100 μm. g The ratio between td-Tomato-positive cell areas and total cell areas of transplanted LGs. n = 7; ***, P < 0.01. h The ratio between lymphatic infiltration areas and total cell areas of transplanted LGs. n = 7; ***, P = 0.3718. i The condition of the NOD/ShiLtJ mice eye orbit before and after injection of ROSA-LGSCs at 8 weeks. There is no decay of the eye orbit on the cell-injected side, whereas there is obvious decay of the eye orbit (red arrow) on the control side. j Tear volume of wild type and NOD/ShiLtJ mice transplanted with ROSA-LGSCs cultured for 7 days after 8 weeks. The tear volume of ROSA-LGSC-injected LGs is significantly higher compared to the control LGs, whereas it is significantly lower compared to WT LGs. WT, wild type, n = 3; NOD/ShiLtJ mice, n = 7; ***, P < 0.01

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