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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair

Fig. 5

Engraftment of NOD-LGSC transplantation and slight relief of ADDED symptoms. a–c IHC staining with anti-mCherry antibody of NOD/ShiLtJ LG transplanted with NOD-LGSCs cultured for 7 days after 8 weeks. a LG injected with vehicle, b LG injected with NOD-LGSCs (red arrow, intralobular duct), c the magnified image of the black frame in b (red arrowhead, acini); scale bar, 100 μm. d The ratio between td-Tomato-positive cell areas and total cell areas of NOD-LGSCs and ROSA-LGSCs transplanted LGs. ROSA-LGSCs transplanted LGs, n = 7; NOD-LGSCs transplanted LGs, n = 3; P = 0.5180. e The ratio between lymphatic infiltration areas and total cell areas of NOD-LGSCs transplanted LGs. n = 3, P = 0.7084. f The condition of the NOD/ShiLtJ mice eye orbit at 8 weeks after injection of NOD-LGSCs. The decay of the eye orbit (red arrow) on the cell-injected side is improved compared to the control side. g Tear volume of wild type and NOD/ShiLtJ mice transplanted with NOD-LGSCs cultured for 7 days after 8 weeks. The tear volume of ROSA-LGSC-injected LGs is significantly higher compared to the control LGs, whereas it is significantly lower compared to WT LGs. WT, wild type, n = 3; NOD/ShiLtJ mice, n = 3; ***, P < 0.01; *, P < 0.05

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