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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Reduced culture temperature attenuates oxidative stress and inflammatory response facilitating expansion and differentiation of adipose-derived stem cells

Fig. 2

Culture of vASCs at 35 °C significantly improves their fat differentiation potential. The fat (A) or bone (B) differentiation capacity of vASCs cultured under 35 °C versus 37 °C was determined by culturing cells, at the respective temperatures, for 21 days in fat or bone differentiation mediums respectively. Mean fat and bone differentiation capacity was assessed by staining cells with Oil red O or Alizarin red respectively, photographing the cells (AI and BI) and then extracting and quantifying the stain (AII and BII) (n = two independent repeats). *p < 0.05. n.s., difference not statistically significant

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Stem Cell Research & Therapy

ISSN: 1757-6512