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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Reduced culture temperature attenuates oxidative stress and inflammatory response facilitating expansion and differentiation of adipose-derived stem cells

Fig. 5

ROS accumulation correlates with the development of a pro-inflammatory phenotype in rat scASCs. Rat scASCs were propagated at 37 °C, and early p ≤ 6 and late p ≥ 10 cells were harvested and analyzed. A ROS accumulation was detected by DCFDA staining and analyzed by flow cytometry at the indicated passages (P). (AI) Representative FACS analyses. (AII) A summary of two independent repeats of the FACS analysis. B Comparison of the percentage of apoptotic cells in early versus late passage of cells grown at 37 °C and 35 °C, as determined by PI/Annexin staining, followed by FACS analysis. (BI) Representative FACS analyses. (BII) A summary of two independent repeats. C RNA was extracted from early and late passages of 37 °C scASCs, and gene expression of the indicated genes was compared by qRT-PCR (n = 2 independent repeats). Data are presented as the mean ± standard deviation. **p < 0.01; *p < 0.05

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