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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Prevention of multiple system atrophy using human bone marrow-derived mesenchymal stem cells by reducing polyamine and cholesterol-induced neural damages

Fig. 5

The effect of polyamine and cholesterol on dopaminergic neurodegeneration and apoptosis in mesenchymal stem cells (MSCs) co-cultured with neuronal cells. The promoting effect of MSCs in terms of PMFBP1 (a) and HMGCL (b) expression were examined using specific antibodies in primary cultured neuronal cells. To assess the involvement of PMFBP1 and the inhibitory effect of MSCs on double-toxin-induced neuro-degeneration, the expression of PMFBP1 was knocked-down in primary cultured neuronal cells using a specific siRNA of PMFBP1 (10 nM) for 24 h at 37 °C. These effects were measured by western blot analysis. The expressions of inflammation marker proteins COX2 and iNOS, apoptotic marker proteins BAX and caspase 3, and TH were examined using specific antibodies (c). To assess the involvement of HMGCL on the inhibitory effect of MSCs on double-toxin-induced neuro-degeneration, primary cultured neuronal cells were treated with recombinant HMGCL protein (10 nM) for 24 h at 37 °C. These effects were measured by western blot analysis. The expressions of inflammation marker proteins COX2 and iNOS, apoptosis marker proteins BAX and caspase 3, and TH were examined using specific antibodies (d). The release of inflammatory cytokines in PMFBP-1-knockdown (e) and HMGCL protein-treated (f) neuronal cells was measured using a specific ELISA kit. To assess the effect of MSCs on polyamine- and cholestrol-induced neuroinflammation, the NO levels (g, i) and expressions of inflammation marker proteins COX2 and iNOS (h and j) were measured. *P < 0.05, significant difference vs. the saline-injected group. #P < 0.05, significant difference among the double-toxin-injected group. $ < 0.05, significant difference between two numbers of MSC-injected group

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