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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Prospective isolation of chondroprogenitors from human iPSCs based on cell surface markers identified using a CRISPR-Cas9-generated reporter

Fig. 5

Histology and IHC for matrix proteins in RVR-COL2 and BJFF pellets. a Safranin-O staining for sGAG showing pellets derived from sorted chondroprogenitor cells had more robust staining and homogenous cell morphology compared to pellets derived from unsorted cells in both lines. b Labeling of COL2A1 showed similar results with an increase in COL2A1 in sorted pellets as opposed to unsorted which has isolated areas of staining. c There was little labeling of COL1A1 for both unsorted and sorted cell pellets. d Labeling for COL10A1 was increased with sorting. Scale bar = 200 μm. Inset scale bar = 400 μm. See also Figure S4 and S5

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Stem Cell Research & Therapy

ISSN: 1757-6512