Fig. 6

Quantitative analysis of matrix production and gene expression. a Sorting of chondroprogenitor cells prior to chondrogenesis significantly increased the sGAG/DNA ratio to approximately 20 ng/ng. b–d Expression of chondrogenic genes ACAN, SOX9, and COL2A1 was significantly increased with sorting. e, f Sorting significantly unregulated fibrocartilage and bone matrix marker COL1A1, and hypertrophic cartilage marker COL10A1. Gene expression in reference to undifferentiated hiPSCs with housekeeping gene TBP. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data represented as mean ± SEM. n = 6–7 per group: 2 experimental replicates, 3–4 technical replicates (pellets). See also Figure S6