Table 2 Primer sequences for RT-qPCR
Gene | Primer sequences |
|---|---|
miR-761 | F: 5′-TTCATTCTACCCAGATGCGTT-3′ |
R: 5′-GAGGATTCACGTGGAGAAGTT-3′ | |
U6 | F: 5′-ATGGGTCGAAGTCGTAGCC-3′ |
R: 5′-TTCTCGGCGTCTTCTTTCTCG-3′ | |
Twist | F: 5′-TACAGCAAGAAATCGAGCGAAG-3′ |
R: 5′-GCTGAGCTTGTCAGAGGGG-3′ | |
LCN2 | F: 5′-ATGTATGGCCGGTACACTCAG-3′ |
R: 5′-AACAAATGCGACATCTGGCAC-3′ | |
E-cadherin | F: 5′-CGTGATGAAGGTCTCAGCC-3′ |
R: 5′-ATGGGGGCTTCATTCAC-3′ | |
N-cadherin | F: 5′-TAGACGAGAGGCCTATCCATGC-3′ |
R: 5′-CAGCAGCTTTAAGGCCCTCAT-3′ | |
Vimentin | F: 5′-AAAGGATCCATGTCTACCAGGTCTGTGTC-3′ |
R: 5′-ACTTCTCAGCATCACGATGACTCTAGATTT-3′ | |
GAPDH | F: 5′-GAAGGTGAAGGTCGGAGTC-3′ |
R: 5′-GAAGATGGTGATGGGATTTC-3′ |