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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mapping molecular pathways for embryonic Sertoli cells derivation based on differentiation model of mouse embryonic stem cells

Fig. 2

Marker identification and transcriptional determination of the cells in the differentiation model. Optical micrographs were displayed on the left. Immunofluorescence (IF) micrographs were on the right. a AMH+ cells were detected. b EMX2+ cells were detected. The cells of a ring-like structure were EMX2−. c SF1+ cells showed more solid than the epithelial-like cells around. dFshr+ cells showed ring-like microstructure. SRY+ cells were detected. e FSHR+ cells showed tubular-like microstructure. f FSHR+ and GDNF+ cells showed tubular-like microstructure. DAPI was a nuclear dye showing blue. Scale bar = 200 μm. The cell portion of g EMX2+ and i FSHR+ cells in differentiation model were determined via IF. The results took a mean value of three parallel experiments (20 views per sample) and were expressed as mean ± SD. The cell portion of h CK18+ and j AMH+ cells in differentiation model was determined via flow cytometry (FCM). Results were expressed as mean ± SD (n = 3 independent experiments). k The five target factors were independently expressed according to the procedures in the differentiation model. The transcriptional expression of target factors was detected through nucleotide band amplified by qPCR in 30 cycles. l Heat map indicated the transcriptional expression of stage-specific markers in the differentiation model by qPCR. Results took the mean value of qPCR (n = 3 independent experiments) and showed changes in gene expression relative to the highest expression in 9–19 days. The multiple ranged from 0 to 10

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