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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mapping molecular pathways for embryonic Sertoli cells derivation based on differentiation model of mouse embryonic stem cells

Fig. 5

Determination of molecular mechanism in maintenance of eSCs. a A schematic diagram of maintenance of eSCs from 12.5 dpc. b Optical micrographs showed ring-like or tubular-like microstructures in different group. c Growth curves of different groups in 12–20 days. Results were expressed as mean ± SD (n = 3 independent experiments). Asterisks indicate statistical significance of difference in the mean cell number calculated by one-way ANOVA method. (*P value < 0.05, **P value < 0.01, ***P value < 0.001). d AMH+ cells were determined via FCM in different groups at 14 days. The control peak took the result of isotype antibody showing a dark color. The positive result refers to the control result. e Optical and IF micrographs were taken in group (W.G.S1.D(constant)) in 20 days. DAPI staining showed blue in cell nucleus. WNT4+ cells showed red. SOX9+ cells showed green. Scale bar = 200 μm. f Nucleotide band of relevant genes was detected at 14 days when Dmrt1 was overexpressed. The band of control group was on the left. The band of test group was on the right. The transcriptional levels of gWnt4 and hAmh were determined in different groups. Results were expressed relative to the highest mean value as mean ± SD (n = 3 independent experiments). Asterisks indicate statistical significance of differences in the mean gene expression calculated by one-way ANOVA method. (*P value < 0.05, **P value < 0.01, ***P value < 0.001)

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