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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Characterization of the immunomodulatory properties of alveolar bone-derived mesenchymal stem cells

Fig. 4

aBMSC and BMSCs did not evoke T cell proliferation. Primary PBMCs prelabeled with CFSE (1 × 105) were cultured in the presence or absence of aBMSCs or BMSCs (1 × 104) with or without a T cell activator for 5 days. Non-adherent cells were harvested and analyzed by flow cytometry using PE-CD4 antibody to label T cells. ad Representative flow cytometric density plots of PBMCs and histograms of CD4+ T cells. a Naïve PBMCs cultured alone without activator. b PBMCs cocultured with BMSCs without activator. c PBMCs cocultured with aBMSCs without activator. d PBMCs cultured alone with activator. e The percentage of divided CD4+ T cells at day 5 of indicated treatment groups. n = 2 for PBMCs cultured alone with or without activator, n = 6 for PBMCs cocultured with aBMSCs from three different donors, and n = 3 for PBMCs cocultured with BMSCs from three different donors

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