Fig. 1

The iPSCs identification. The iPSCs showed embryonic stem cell-like morphology during expansion (a) and exhibited positive alkaline phosphatase staining (b). EBs were formed (C) and able to differentiate into three germ layer cells (d). The iPSCs displayed obvious immunofluorescence of pluripotent proteins OCT4, NANOG, and TRA-1-60 (e). The expression of pluripotent genes (Oct4, Nanog, Sox2) and three germ layers genes (Nestin, Map 2, Desmin, Msx1, Sox17) were assessed in iPSCs and EBs (f). Scale bar, a and b 100 μm; c–e 200 μm. Data presented as mean ± SEM. ^##p < 0.01; ^###p < 0.001