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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy

Fig. 6

The hypertrophic differentiation of iPSC-derived chondrocytes with different concentrations of L2C4S4/ TCP extracts in 14 days. a Immunofluorescence of hypertrophic specified protein COLX and MMP13. b The average fluorescence intensity of COL X and MMP13 protein in each group, n = 5. c RT-PCR of hypertrophic genes Col10a1, Mmp13, and Ihh, n = 3. Data presented as mean ± SEM. Scale bar = 200 μm. CTR: MCDM without any extracts. #p< 0.05; ##p < 0.01, *p < 0.05, **p < 0.01

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