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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Priming with inflammatory cytokines is not a prerequisite to increase immune-suppressive effects and responsiveness of equine amniotic mesenchymal stromal cells

Fig. 7

Photomicrographs representative of three different experimental conditions: LPS (lane A), LPS-CM-AMSC (lane B), LPS-CM-TNF-α (lane C), LPS-CM-IFN-γ (lane D), and LPS-CM-TNF-α+IFN-γ (lane E). Corresponding epifluorescence images showing cell nuclei (column 1: Hoechst 3258) and epifluorescence images showing mitochondrial activity (column 2: MitoTracker Orange) and intracellular ROS levels (column 3: H2DCF-DA). A slight increase of mitochondrial activity and intracellular ROS levels can be seen in the LPS-exposed cells (F2, F3) compared with the other conditions. Scale bars represent 20 μm

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