Table 1 Oligonucleotide sequences used for RT-PCR analysis
Markers | Sequences (5′➔3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | S: AGATCAAGAAGGTGGTGAAG A: TTGTCATACCAGGAAATGAGC | 168 | 60 |
Major histocompatibility complex I (MHC-I) | S: GGAGAGGAGCAGAGATACA A: CTGTCACTGTTTGCAGTCT | 218 | 55 |
Major histocompatibility complex II (MHC-II) | S: TCTACACCTGCCAAGTG A: CCACCATGCCCTTTCTG | 178 | 55 |