Fig. 6

EVs potentiated the angiogenic activities of ECs in vitro. a Representative images of the tube formation assay in ECs treated with H-EVs, P-EVs, and an equal volume of PBS (scale bar, 200 μm). b Quantitative analyses of the covered area, total branching points, total loops, and total tube length in b (n = 3). c The proangiogenic genes VEGF and AngII in ECs were analyzed by qRT-PCR analysis (n = 3). d Detection of the protein levels of VEGF and AngII in ECs by western blot analysis. e Quantitative analysis of the relative protein expression (n = 3). *P < 0.05, **P < 0.01 vs. the control group; ^#P < 0.05, ^##P < 0.01 vs. the H-EV group