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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Tumor necrosis factor α in aGVHD patients contributed to the impairment of recipient bone marrow MSC stemness and deficiency of their hematopoiesis-promotion capacity

Fig. 4

TNF-α in aGVHD BM supernatants contributed to the impairment of the self-renewal of MSCs. a The self-renewal activities of MSCs were assayed by CFU-F experiments and sphere formations in the presence of neutralization antibodies (anti-TNF-α, anti-IL-17A, anti-IL-1β, and anti-IL-2) and aGVHD BM supernatants. Bar in a upper row represents 5 mm. Bar in a lower row represent 200 μm. b The CFU-F number and c the cell sphere number in the TNF-α neutralization group were significantly higher than that of no antibodies group. **p < 0.01, n = 5. d The neutralization of TNF-α increased the mRNA expression of Oct-4 and Sox-2 in MSCs. **p < 0.01, compared with aGVHD groups, n = 5

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