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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MSC-derived exosomes attenuate cell death through suppressing AIF nucleus translocation and enhance cutaneous wound healing

Fig. 1

Characterization of human umbilical cord mesenchymal stem cells (hucMSC) and hucMSC-derived exosomes (hucMSC-Ex). a, b Low cytometry and immunofluorescence of phenotypic markers of MSCs. The hucMSC were positive for CD44, CD73, CD90, and CD105 and negative for CD34 and CD45. Scale bar = 200 μm. c Cell lineage-induced differentiation. Adipogenic, osteogenic, and chondrogenic differentiations were analyzed by Oil-Red-O, Alizarin Red, and Toluidine Blue staining, respectively. Scale bar = 100 μm. d Representative TEM micrographs of purified spheroid hucMSC-Ex. Scale bars = 200 nm. e Western blot indicated positive CD9, CD81, Alix, TSG101, and HSP70 protein expression in hucMSC-Ex. f Size distribution of hucMSC-Ex. g The PKH26-labeled exosomes (red) can go into HaCaT cell (blue)

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Stem Cell Research & Therapy

ISSN: 1757-6512