Fig. 3

Changes of apoptotic proteins in HaCaT after H₂O₂ treatment. a Protein changes in mitochondria. b Compared to 0h, AIF and cyt C were downregulated and calpain 1 was upregulated following H₂O₂induction. c Protein changes in the nuclei. d Compared to 0 h, AIF, PARP-1, and CypA were upregulated following H₂O₂induction. e Protein changes in the cytosol. f, g Compared to 0 h, Hsp70 was downregulated while caspase-3 and cyt C were upregulated. h PAR changes in the cytosol. i Compared to 0 h, PAR was upregulated, reaching plateau at 6-8 hour after H₂O₂ treatment. Relative to 0 h: ^*P < 0.05 and ^**P < 0.01. Data are representative of three independent experiments (means ± SD)