Fig. 5
HucMSC-Ex inhibited H2O2-induced apoptosis and promoted proliferation of HaCaT cells. a The CCK-8 assay showed that the viability of the cells in the hucMSC-CM group was higher than that for the cells in the PBS group. b HaCaT cells were treated with H2O2 then cultured with hucMSC-CM, hucMSC-dp-Ex, or hucMSC-Ex for 24 h. Viability of the cells in hucMSC-Ex was higher than those for the control and hucMSC-dp-Ex groups. c, d HucMSC-Ex promoted HaCaT proliferation in a dose- and time-dependent manner. e HucMSC-CM and hucMSC-Ex restored normal HaCaT cell morphology after H2O2 induction whereas the other treatments could not. f, g Flow cytometry showed that hucMSC-Ex reduced the fluorescence intensity of HaCaT cells induced with H2O2. h, i Flow cytometry showed that hucMSC-Ex inhibited apoptosis of HaCaT cells induced with H2O2. Compared to control: *P < 0.05 and **P < 0.01; compared to H2O2:#P < 0.05 and ##P < 0.01. Data are representative of three independent experiments (means ± SD)