Fig. 3

MSC-MVs restore the BLM-inhibited autophagy via transferring miR-100 targeting mTOR. a The putative miR-100 binding sites in mTOR (mTOR WT) or and the designed mutant sequence (mTOR Mut) were indicated. Luciferase reporter assay was conducted to evaluate the interaction between mTOR 3′-UTR and miR-100. b The protein levels of mTOR and autophagy-related LC3-I, LC3-II, and Beclin-1 in L2 cells in the groups of control, BLM, BLM+MVs, BLM+NCI-MVs, and BLM+miR-100I-MVs. Data are presented as the means ± SD (n = 3). **p < 0.01, vs. mimic NC; ^##p < 0.01, vs. control; ^$p < 0.05, ^$$p < 0.01, vs. BLM; ^&p < 0.05, ^&&p < 0.01, vs. BLM+NCI-MVs