| DF6 | CB6 | BM1 | IMR90 | FR202 |
---|---|---|---|---|---|
(A) Cardiac differentiation efficiency with CHIR99021 in MNL cultures (Maximum flow cytometry population expression at 4-14 μM CHIR99021 on day14) | |||||
NKX2–5 (%) | 21.8 ± 17.1 | 1.7 ± 0.1 | 78.8 ± 25.5 | 82.9 ± 8.4 | 57.1 ± 7.2 |
Troponin T (%) | 29.7 ± 24.6 | 2.1 ± 0.4 | 81.0 ± 31.2 | 83.1 ± 8.9 | 80.6 ± 2.1 |
MLC2a (%) | 34.9 ± 25.7 | 1.95 ± 0.3 | 70.4 ± 21.9 | 64.9 ± 0.1 | 64.9 ± 9.4 |
CD44 (%) | 40.5 ± 9.2 | 32.1 ± 8.4 | 16.5 ± 11.7 | 37.1 ± 14.9 | 3.7 ± 3.7 |
HNF4a (%) | 38.8 ± 14.8 | 7.4 ± 1.9 | 13.6 ± 1.5 | 20.7 ± 5.9 | 4.4 ± 4.4 |
(B) Cell growth on MC in stirred spinner cultures (day 6) | |||||
Cells/mL (×106) |  | No cell growth | 1.7 ± 0.3 | 1.9 ± 0.6 | |
Expansion fold | 14.0 ± 0.2 | 16.0 ± 0.5 | |||
Aggregate size (mm2) | 0.42 ± 0.1 | 0.30 ± 0.1 | |||
Oct4a | 94.3 ± 1.1 | 91.0 ± 0.1 | |||
Tra-1-60 | 93.0 ± 0.01 | 96.4 ± 0.1 | |||
(C) Cardiac differentiation on MC in stirred spinner cultures (day 12 of differentiation) | |||||
Cells/mL (×106) |  | 2.1 ± 0.4 | 2.3 ± 0.2 | ||
Troponin T (%) | 14.4 ± 8.5 | 83.2 ± 0.13 | |||
CM yields (cells/mL × 106) | 0.32 ± 0.2 | 1.9 ± 0.03 |