Fig. 1

Clonal complexity declines during the culture of primary MSCs. a Schematic presentation of the experimental workflow. Lentiviral transduction (LV-trans.) with RGB-BC LeGO vectors, puromycin selection, culture expansion, and sampling of primary MSCs. P, passage; d, day. b Exemplary fluorescence microscopy image of RGB-marked MSCs. c–e Sequencing results of barcodes (colors indicate the corresponding fluorochrome of the vector construct). The contribution of each specific barcode is represented as a percentage of the total number of barcode reads. Changes were analyzed at consecutive passages for each donor. f Shannon Index indicates decline of subclone-diversity during culture expansion of MSCs