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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells

Fig. 2

Expression analysis of germ cell specific genes during hPGCLC specification process. a Expression dynamics of germ cell specific genes during hPGCLC specification process, including iPSCs, iMeLCs, and the whole floating embryoids at day 2, 4, 6, and 8 of induction, as measured by qRT-PCR. Relative expression levels are shown with normalization to housekeeping gene GAPDH. Error bars indicate mean ± SD of three independent experiments. Red squares indicate values for embryoids exposed to cytokine stimulation; black triangles indicate values for embryoids formed spontaneously without cytokine stimulation. *p < 0.05 vs. the iPSC groups. n.d., not detected. b Immunofluorescence analysis for the expression of TFAP2C, PRDM14, OCT4, and NANOS3 in day 4 embryoids derived from hiPSCs. Scale bars, 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512