Fig. 4

T1D ASCs promote wound vascularity and collagen I expression. H&E staining of wound sections of normal control group treated with PBS (A), T1D mice wounds treated with PBS (B), ASCs (C), or T1D ASCs (D) 14 days post-wounding. CD34 (red arrows) immunohistochemistry staining of wound sections of normal control group treated with PBS (E); T1D mice wounds treated with PBS (F), ASCs (G), or T1D ASCs (H) 14 days post-wounding. Collagen I protein immunohistochemistry staining of wound sections of normal control group treated with PBS (I); T1D mice wounds treated with PBS (J), ASCs (K), or T1D ASCs (L) 14 days post-wounding. Bars = 100 μm. M Gene expression analysis by qRT-PCR using actin as the endogenous control. *p < 0.05, **p < 0.01, ***p < 0.001 vs. PBS controls. Triplicate results were averaged and mean ± SD is shown, ANOVA test