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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

Fig. 2

Culture and identification of hDPSCs. a Primary cultured hDPSCs (× 100). b A single cell was obtained (× 100). c Single cell-derived colonies were obtained after culture for 14 days (× 40). Alizarin Red staining was performed to detect mineral nodes in the control group (d) and experimental group (e) (× 100). f–k Flow cytometric analysis of the surface markers of hDPSCs. Cells were incubated with fluorescently conjugated antibodies against CD29, CD90, CD44, CD105, CD34, and CD45. Isotype-identical antibodies served as controls. Analysis of molecular surface antigen markers in hDPSCs by flow cytometry indicated that the cells were positive for CD29, CD44, CD90, and CD105 and negative for CD34 and CD45

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