Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells

Fig. 1

Characterization of BM-MSC before transplantation into hydrocephalic hyh mice. a BM-MSC primary culture forming a colony unit. Phase-contrast microscopy. b BM-MSC expressing the mRFP1 (red). Merge of phase-contrast and epifluorescence microscopies. c Spindle-shaped BM-MSC under scanning electron microscopy. d Oil Red O staining of lipid droplets (arrows) in the cytoplasm of BM-MSC after adipogenic differentiation for 14 days. Merge of phase-contrast and bright-field microscopy images. e Collagen type II immunostaining of a 3D pellet culture of BM-MSC after 21 days of chondrogenic differentiation. f Osteoinducted BM-MSC stained with alkaline phosphatase (ALP) 21 days after induction. g ALP activity in control (black) and osteoinducted (gray) BM-MSC at 7, 14, and 21 days. h, i Representative immunophenotype profiles of unfixed BM-MSC for CD44 and CD34 markers by flow cytometry. j Flow cytometry analysis of DNA content (DAPI fluorescence) in fixed BM-MSC. k, l Detection of a neural cell marker (GFAP) and a neuroprotector factor (GDNF) in fixed BM-MSC by flow cytometry. m Immunofluorescence (green) in fixed BM-MSC before injection for δGFAP, nestin, NG2, αGFAP, β-III tubulin, NeuN, GDNF, NGF, BDNF, and VEGF. In the absence of labeling, the channel for red (RFP fluorescence) is also shown. Nuclear staining with DAPI (blue). Negative control represents the omission of the primary antibodies. **P < 0.02, ***P < 0.01 Wilcoxon-Mann-Whitney test

Back to article page