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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: A model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatment

Fig. 2

Colony-forming ability, population doubling property, and chromosomal stability of ten donor-derived hDPSCs. a, b The colony-forming ability of the ten donor-derived hDPSCs was tested by CFU-F assay. Representative images of the CFU-F are shown by toluidine blue staining (a). The average number of colonies is shown (b). c Population doubling assay is analyzed by sequential cell passaging every 7 days. The results are shown as the average total population doubling levels (PDL). PDL were shown at each passage until the ten donor-derived hDPSCs lost their proliferation capacity. d Chromosomal stability was tested by G-band karyotype assay in the P10 hDPSC products. Donor #3-derived hDPSCs were excluded because of the very low PDL. A chromosomal aberration was detected in donor #8- and #9-derived P10 hDPSC. N/A, not applicable

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