Fig. 2From: A model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatmentColony-forming ability, population doubling property, and chromosomal stability of ten donor-derived hDPSCs. a, b The colony-forming ability of the ten donor-derived hDPSCs was tested by CFU-F assay. Representative images of the CFU-F are shown by toluidine blue staining (a). The average number of colonies is shown (b). c Population doubling assay is analyzed by sequential cell passaging every 7Â days. The results are shown as the average total population doubling levels (PDL). PDL were shown at each passage until the ten donor-derived hDPSCs lost their proliferation capacity. d Chromosomal stability was tested by G-band karyotype assay in the P10 hDPSC products. Donor #3-derived hDPSCs were excluded because of the very low PDL. A chromosomal aberration was detected in donor #8- and #9-derived P10 hDPSC. N/A, not applicableBack to article page