Fig. 3

Characterization of the properties of P3 hDPSC products at freezing for the MCB. P3 hDPSC products were independently produced by the passage and expansion of the CFU-F-formed cells from ten donors. Characterization of the properties of the P3 hDPSC products was carried out before MCB storage. a Morphology of the P3 hDPSCs is shown as the representative microscopic images of each donor. Bars = 100 μm. b Cell surface marker analysis of the P3 hDPSCs was tested by flow cytometric assay. The results are shown as the average positive rates of each marker. c Multipotency of the P3 hDPSCs was assessed by the differentiation-specific gene expression of human osteoblasts, adipocytes, and chondrocytes in the P3 hDPSCs 1, 6, and 4 weeks after the induction, respectively, quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. The results are shown as the ratios of the expression of 18S and are shown as the average ratio. ALP, alkaline phosphatase gene; BGLAP, bone gamma-carboxyglutamine protein gene; COL10, collagen type X gene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase gene; LPL, lipoprotein lipase gene; PPARG2, peroxisome proliferator activated receptor gamma 2 gene; RUNX2, runt-related transcription factor 2 gene; SOX9, SRY-box 9 gene. c Secretion of anti-inflammatory factors from the P3 hDPSCs was tested by enzyme-labeled immunosorbent assay (ELISA). The results are shown as the average concentration of hepatocyte growth factor (HGF), interleukin 6 (IL6), monocyte chemotactic protein 1 (MCP1), and sialic acid-binding immunoglobulin-type lectin 9 (SIGLEC9) in the conditioned medium of each donor. b–d The graph bars show the mean. n = 3 for all groups