Fig. 4

Characterization of the properties of P10 hDPSC products for the WCB. P10 hDPSC products were manufactured from cryopreserved hDPSC products in the MCB. Donor #3-, #8-, and #9-derived hDPSC products were discarded because of the low proliferation and chromosomal aberrations, as shown in Fig. 1. The other seven donor-derived P10 hDPSC products were characterized before WCB cryopreservation. a Morphology of the P10 hDPSCs is shown as a representative microscopic image. Bars = 100 μm. b Cell surface marker analysis of each donor P10 hDPSCs was tested by flow cytometry. The results are shown as the average positive rates of each marker. c Multipotency of the P10 hDPSCs was assessed by the differentiation-specific gene expression of human osteoblasts, adipocytes, and chondrocytes 1, 6, and 4 weeks after the induction, respectively, by qRT-PCR assay. The results are shown as the ratios of the expression of 18S and are shown as the average ratio. d Secretion of anti-inflammatory factors from the P10 hDPSCs was tested by ELISA. The results are shown as the concentration of each factor in each donor. a–d N/A, not applicable. b–d The graph bars show the mean. n = 3 for all groups