Fig. 7
Transplantation of P10 hDPSC products improved chronically CCl4-damaged liver dysfunction in mice. Each donor-derived P10 hDPSC product was intrasplenically transplanted into 4-week-CCl4-treated immunocompetent mice without immunosuppressant (n = 5). The age-matched control non-CCl4-treated (n = 5) and non-transplanted CCl4-treated (n = 5) immunocompetent mice were used as experimental controls. a–d Distribution of the donor cells in the CCl4-damaged fibrotic liver tissues was investigated by immunohistochemical assay. The results are shown as the representative microscopic images using anti-HLA-ABC (a) and anti-hepatocyte paraffin 1 antigen (HepPar1) (b) antibodies. Nuclei were stained with hematoxylin. Bars = 50 μm (a, b). The results are shown as the ratios of the HLA-ABC- (c) and HepPar1- (d) positive areas in the mouse liver tissue. e–h Serum levels of aspartate aminotransferase (AST, e), alanine aminotransferase (ALT, f), total bilirubin (g), and human albumin (ALB, h) were examined by biochemical assays and ELISA. a–h N/A, not applicable. c–hn = 5 for all groups. *P < 0.05, ***P < 0.005. The graph bars show the mean ± SEM (white columns) or the mean (black columns). ND, not detected; ns, not significant