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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: A model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatment

Fig. 8

Transplantation of P10 hDPSC products improves CCl4-induced liver fibrosis in mice. Each donor-derived P10 hDPSC products were intrasplenically transplanted into 4-week-CCl4-treated immunocompetent mice without immunosuppressants (n = 5). The age-matched control non-CCl4-treated (n = 5) and non-transplanted CCl4-treated (n = 5) immunocompetent mice were used as experimental controls. a–c Expression of the activated stellate cells in the CCl4-damaged fibrotic liver tissues was investigated. Representative immunohistochemical images show the distribution of actin alpha 2 smooth muscle, aorta (ACTA2) in mouse liver tissue. Nuclei were stained with hematoxylin. Bars = 50 μm (a). The area of ACTA2-positive cells in mouse liver tissue was analyzed (b). The expression of the Acta2 was investigated by qRT-PCR (c). d–f Deposition of fibrous tissues in CCl4-damaged liver tissues was investigated. Representative histological images show the distribution of fibrous tissue in mouse liver tissue by picrosirius red staining. Bars = 50 μm (d). The picrosirius red-positive area in mouse liver tissue was analyzed (e). The expression of the collagen type I alpha 1 chain gene (Col1a1) was investigated by qRT-PCR. The results are shown as the ratios of the expression of 18S (f). The liver fibrosis stages were assessed by Ishak scoring (g). a–g N/A, not applicable. b, c, e–gn = 5 for all groups. *P < 0.05, ***P < 0.005. The graph bars show the mean ± SEM (white columns) or the mean (black columns). ns, not significant

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