Fig. 4

LAMA2 promoted adipogenic differentiation of MSCs in vitro and in vivo. a Knockdown of LAMA2 inhibited adipogenesis as indicated by Oil Red O staining. b Western blot (left) showed that the protein expression of PPARγ was upregulated in the LAMA2 knockdown group compared with the control group (NC) after 21 days of adipogenic induction. Western blot quantification (right) showed the same result. c Suppressing the expression of LAMA2 inhibited the mRNA expression of PPARγ and C/EBPα, as detected by RT-qPCR. d H&E staining illustrated that LAMA2 knockdown groups had fewer adipose tissue-like constructs in vivo. e Oil Red O staining illustrated that LAMA2 knockdown groups had fewer adipose tissue-like constructs in vivo. *p < 0.05, **p < 0.01