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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Targeting prostate cancer stem-like cells by an immunotherapeutic platform based on immunogenic peptide-sensitized dendritic cells-cytokine-induced killer cells

Fig. 4

Detection of apoptotic cells in prostatic cells upon 1 h incubation with P-DC-CIK or NP-DC-CIK by TMR red-based TUNEL assay. a Representative images show the TUNEL-positive apoptotic cells induced in prostatospheroid-derived prostate cancer cells. b Graph shows the quantitation of TMR red signals detected in prostatic cells upon co-cultures with P-DC-CIK or NP-DC-CIK cells. Results showed that significant increases of TMR red-labeled apoptotic cells were detected in prostatospheroid-derived prostate cancer cells (DU145, LNCaP, and VCaP) upon co-culture with the P-DC-CIK as compared to the NP-DC-CIK control. Results also showed that no TMR red-labeled apoptotic cells were detected among BPH-1 immortalized non-malignant prostatic epithelial cells upon incubation with either P-DC-CIK or NP-DC-CIK. Magnification, × 20; bars, 100 μm. NP-DC-CIK non peptide-sensitized dendritic cells-cytokine-induced killer cell preparation, P-DC-CIK peptide-sensitized dendritic cells-cytokine-induced killer cell preparation

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Stem Cell Research & Therapy

ISSN: 1757-6512