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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Exosomes derived from human umbilical cord mesenchymal stem cells inhibit vein graft intimal hyperplasia and accelerate reendothelialization by enhancing endothelial function

Fig. 5

hucMSC-exosomes promoted in vitro proliferation and migration of HUVECs in a dose-dependent manner. HUVECs were incubated in the presence of different concentrations of hucMSC-exosomes (25, 50, and 100 μg/ml) for 24 h. a Representative images of the EdU incorporation assay of HUVECs. EdU-positive cells were red-stained, and negative cells had blue nuclei (Hoechst 33342). Bar, 50 μm. b Quantification of proliferation rates (percentage of EdU-positive cells) of HUVECs showed that the proliferative capacity of HUVECs was significantly improved dose-dependently. c Representative images of the scratched wound assay. Bar, 200 μm. d Quantitative analysis of the migration area of HUVECs showed that the migratory capacity of HUVECs was significantly improved dose-dependently. e Representative images of the Transwell assay of HUVECs. Bar, 100 μm. f Quantification of the number of migrated cells HUVECs showed that the migratory capacity of HUVECs was significantly improved dose-dependently. The results are presented as the mean ± SD, n = 3 for each group. An asterisk represents statistically significant difference compared with the control group (P < 0.05)

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